In vitro establishment of two eucalyptus hybrids under different concentrations of agar
Ari Medeiros Braga Neto, Lauana Lopes dos Santos, Ramony Cristina Lima, Márcio Takeshi Sugawara, Bruno Oliveira Lafetá, Alisson José Eufrásio de Carvalho, Enilson de Barros Silva
Journal Title:Revista Agraria Academica / Agrarian Academic Journal
The objective of this work was to evaluate the in vitro establishment of two eucalyptus hybrids (Eucalyptus urophyllax Eucalyptus globulus and Eucalyptus urophyllax Eucalyptus grandis) under different concentrations of agar in order to obtain a concentration that provides better performance of the hybrids. The in vitro establishment was performed in a completely randomized experimental design with four replicates, in the factorial scheme 4 x 2, and the effect of four bacteriological agar concentrations (0, 3,6 and 12 g L-1) on two eucalyptus hybrids, in MS culture medium. At 30 days after inoculation, the presence of leaves, petiole, bud, callus, oxidation of the medium, explant oxidation and the incidence of Fusarium sp., Aspergillus sp. and bacteria were recorded. The concentration of agar that provided the best establishment of the explants is in the range of 7,25 to 7,94 g L-1of agar.