Cytotoxic Potentials of Clerodendrum Volubile against Prostate Cancer Cells and Its Possible Proteomic Targets
Saheed Afolabi , Olufunke Olorundare , Gideon Gyebi , Deeba N Syed , Hasan Mukhtar, Ralph Albrecht and Mamoru Koketsu
Journal Title:Journal of Clinical Nutrition and Food Science
The plant Clerodendrum volubile consumed by the Niger/Delta people of Nigeria has additional therapeutic applications for diabetes, ulcer, arthritis, rheumatism, dropsy, etc. The present study was conducted on the methanol extracts from Clerodendrum volubile leaves (CVE), with the aim of exploring its cytotoxic activity against prostate cancer (PCa) cells and delineate its potential mechanistic targets. CVE inhibited cell viability and suppressed clonogenic potential of PCa cells as assessed by MTT and colony assays. Western blot analysis of CVE-treated cells revealed concentration-dependent cleavage of PARP and Caspase 3. Flow cytometric data validated apoptosis as the primary mechanism of CVE-induced cell death with significant increase in the number of apoptotic cells subsequent to treatment. Furthermore, CVE-treated cells demonstrated increased levels of cyclin- dependent kinase inhibitor p21, suggesting modulation of cell cycle machinery. Profiling of CVEtreated PCa cells, utilizing a LC-MS/MS based quantitative proteomic strategy, yielded a data set of 16 significantly modulated proteins. Other than three proteins, ALBU, ROA3 and HNRPC that were downregulated [1.7; 1.7 and 2.5 fold, respectively], 13 proteins including TALDO, SYWC and FUMH with specific roles in the pentose phosphate pathway and tricarboxylic acid cycle were significantly upregulated [2.4; 1.9 and 1.8 fold, respectively] in CVE-treated PCa cells. Our studies provide insight into plausible mechanism(s) through which CVE exerted its growth inhibitory effect against PCa cells. Further studies are warranted to characterize the effect on functional pathways modulated by CVE with special focus on glucose metabolism.