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Paper Details

Molecular Identification of Etiological Agents of Chromoblastomycosis in Costa Rica

Daniela Jaikel-Víquez, Stefany Lozada-Alvarado and Lorena Uribe-Lorío

Journal Title:Acta Scientific Microbiology
Abstract


Chromoblastomycosis is the second most frequently reported subcutaneous mycosis in Costa Rica. It is caused by dematiaceous fungi belonging to the family Herpotrichiellaceae (Order Chaetothyriales), especially by Fonsecaea pedrosoi and Cladophialophora carrionii. However, it is important to note that Fonsecaea monophora is able to disseminate and cause cerebral phaeohyphomycosis. Thus, five clinical isolates deposited in the Fungal Collection of the School of Microbiology of the University of Costa Rica were ana-lyzed. The isolates were characterized macroscopically and microscopically after grown in potato dextrose agar. Genetic identifica-tion was performed via amplification and sequencing of the ITS (internal transcription spacer) region. The isolates were identified as F. pedrosoi (n = 3), F. monophora (n = 1) and Rhinocladiella aquaspersa (n = 1). Hence, we report for the first time that R. aquaspersa is an etiological agent of chromoblastomycosis in Costa Rica and confirm the presence of both F. pedrosoi and F. monophora in the country. Therefore, we recommend the usage of molecular techniques to identify these pathogens since there is a risk of fungal dis-semination in our patients

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