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Paper Details

Culture Parameters Affect the Light Emitting Property of Organisms Isolated from Two Marine Fishes

Anuradha Pandey Dubey and Madhuri Sharon

Journal Title:Acta Scientific Microbiology

Organisms were isolated and cultured from two marine fishes (Stolephorus indicus and Nemipterus japonicus) that were exhibiting Bioluminescence. Identification and assessment of impact of five different culture media on the growth and light emission properties were studied. Isolates from both the fishes showed microbes to be gram negative Coccobacilli, Partial gene sequencing analysis sug-gested that the microbes belonged to Vibrio spp and Photobacterium spp. Biochemical analysis of both the bacterial isolates exhibited difference in sugar (Mannitol and Lactose), LDC, Indole and Urea content; suggesting that the two isolated colonies are different bio-luminescent bacteria. Repeated subculture (to obtain pure colonies) of both the isolates resulted in gradual reduction in the intensity of luminescence of the bacteria and eventually loss of luminescence property occurred. However, luminescence was revived when they were grown in aerated condition