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Impact of Oocytes Insemination Timing on Fertilisation and Embryo Development Rate

Chandan N*, Raghunandan K, Reeta Janet Jessy I, Saleem M and Shwetha C

Journal Title:Acta Scientific Women's Health

Background: Pre-incubation period between oocyte retrieval and injection in Intracytoplasmic sperm injection (ICSI) cycles improved the percentage of mature oocytes, the preincubation of oocytes prior to in vitro fertilization (IVF) improves the fertilization rate and the quality of embryo formation. Objective: The study was to analyze whether the preincubation of oocytes and microinjection timings on the outcome of ICSI increases rates of fertilization and the quality of embryo formation and also to evaluate the relationship between different pre-incubation periods of oocytes. Design: Retrospective randomized controlled trial. Methods: A total of 129 patients undergoing their Intracytoplasmic sperm injection cycles, retrieved oocytes were preincubated for 2 - 6h prior to injection, the injected oocytes were cultured in Culture NX media, fertilization and Embryo development assessment was performed on day 1 and day 3, Patients were randomly divided into different groups. Results: Neither 2 - 3 hour and 3 - 4 hour insemination time of post oocyte retrieval had no significant influence on the fertilisation rate (FR) and showed no significant improvement in Embryo development, fertilization rate showed a significant improvement after inseminating at 2 - 3 hour gives 86% fertilization rate and 95% embryo development rate while FR significantly decreased with the increase of insemination time. Also, inseminating oocytes at 3 - 4 hour post oocyte retrieval gives 81% fertilization rate and 97% embryo development rate.