SELECTED RENAL OXIDATIVE STRESS MARKERS AND LIPID PROFILE CHANGES DUE TO LONG TERM CONSUMPTION OF INSTANT NOODLES IN ADULT WISTAR RATS
Garba Abdullahi, Adjene Josiah Obaghwarhievwo and *Odigie Mike Osagie
Journal Title:European Journal of Pharmaceutical and Medical Research
Reports have linked the levels of renal antioxidant markers to the development of kidney disease. Though the effects of instant noodles consumption on the levels of these markers and serum lipid profile remain vague and poorly established, this study sought to investigate the long term consumption of instant noodles on selected oxidative stress markers and lipid profile levels of Wistar rats. Fifty (50) adult wistar rats of an average weight of 200g were purchased for the study. After fourteen (14) days of acclimatization, the rats were then grouped into five (5) groups of ten (10) rats each. While Group A was fed with cooked instant noodle (Type A), group B received cooked instant noodles mixed with its spice, whereas, group C and D were respectively fed with cooked instant noodles (type B) and type B with spice. Lastly, group E (Control) rats received standard rat chow and the other animals were given water ad libitum. At the end of administration of test substances, rats were sacrificed (by cervical dislocation) and kidney tissue harvested and homogenized for assay of selected oxidative stress markers [Catalase, Malonyldialdehyde (MDA), Glutathione Peroxidase (GPx) and superoxide Dismutase (SOD)]. Blood sample was also collected and subjected to biochemical analysis of various levels of lipid profile [Triglycerides (TG) Total Cholesterol (TC), High Density Lipoprotein (HDL), and Low Density Lipoprotein (LDL)]. After data collation, result showed a statistically insignificant increase (p < 0.05) in serum lipid levels of all treated animals as against those of the control group. Renal antioxidant enzyme levels, we observed a statistically significant (p < 0.05) decrease in Catalase, SOD and GPx concentrations in the treatment group. However there was a significant (p < 0.05) increase in MDA levels in the treatment groups as compared with control, which was a strong indication that renal cortical structures were compromised. Study also observed a statistically significant (p < 0.05) increase in all assayed lipid profiles levels. We strongly recommend further studies aimed at corroborating these findings.