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Paper Details

GC-MS ANALYSIS OF PHYTOCONSTITUENTS AND CONCURRENT DETERMINATION OF FLAVONOIDS BY HPLC IN ETHANOLIC LEAF EXTRACT OF BLEPHARIS MADERASPATENSIS (L) B. HEYNE EX ROTH

M. Suriyavathana* and S. Indupriya

Journal Title:World Journal of Pharmaceutical Research
Abstract


Objectives: To elucidate the phytochemical constituents using GC-MS and a sensitive HPLC method has been developed for the concurrent determination of five major flavonoids in ethanolic leaves extract of Blepharis maderaspatensis. Methods: The shade-dried leaves of B. maderaspatensis were extracted with ethanol, the concentrated ethanolic extracts were further subjected to GC-MS and HPLC. Results: The GC-MS analyses determined the presence of 17 different phytochemical compounds in the ethanolic leaves extract of B. maderaspatensis. The phytoconsituents compounds were found in the mass spectra was matched with the National Institute of Standards and Technology (NIST) library. The major phytoconstituents are 9-Eicosyne (23.64%), Sqalene (11.51%), Phytol (8.40%), 3,4-Dihhdro-3,5,8-trimethyl-3-(4,8,12trimethyltridecyl)-(2H)-benzopyran-6-acetate (8.07%), 3,7,11,15 Trimethyl-2-hexadecen-1-ol (7.41%) and Cholestan-3-ol, 2 methylene,(3,5) (7.24%). Mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. The Phytochemical profile of five flavonoids contents in the ethanolic leaf extract of B. maderaspatensis are Gallic acid (16.0 g/g), Caffeic acid (0.1 g/g), Rutin (0.1 g/g), Quercetin (0.2 g/g) and Ferulic acid (0.01 g/g). Conclusions: This is the first report of documentation of active constituents from leaves of B. maderaspatensis. The results of the present study reveal that the leaves of B. maderaspatensis indulge effective potential bioactive compounds, which may be leads to the formulation of new drugs to treat various diseases.

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