EFFECT OF CHLORINE DIOXIDE ON ENTEROCOCCUS FAECALIS IN EXPONENTIAL, STATIONARY AND STARVATION PHASES OF EXTRACTED HUMAN TEETH - AN IN VITRO STUDY
Shirur Krishnaraj SOMAYAJI, Shobha KL, Mohandas RAO KG
Journal Title:International Journal of Medical Dentistry
Enterococcus Faecalis (E. faecalis) is usually isolated from failed root canal-treated teeth. It can bind to dentin and co-aggregate with other organisms. The organism is resistant to intracanal medicaments and irrigants. It has the ability to produce biofilms and survive within the root canal and it may develop resistance in different phases of the bacterial growth cycle.
Objective: To study the effect of chlorine dioxide on different phases of E. faecalis growth cycle for 1 and 3 min durations.
Methodology: E. faecalis ATCC strain (29212) was grown in different culture phases on dentinal blocks of extracted human teeth. After treatment with chlorine dioxide, the left out colonies were counted from dentinal shavings. Scanning electron microscopic observations of the remaining biofilm in different phases were made and compared among them.
Results: In all 3 phases, in the end of the 1 and 3 min time, significantly fewer (p