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Paper Details

MOLECULAR CHARACTERIZATION OF JASMINE SPECIES BY RAPD MOLECULAR MARKERS

Sushant Shekhar and Prasad M.P*

Journal Title:World Journal of Pharmaceutical Research
Abstract


In the following study genomic DNA was extracted from eight Jasmine species. DNA fingerprinting was performed by the random amplified polymorphic DNA (RAPD)-PCR. RAPD has been one of the most commonly used molecular techniques to develop DNA markers since it does not require prior knowledge of a DNA sequence. RAPDPCR produced a spectrum of amplification products which are characteristics of the selected Jasmine DNA. From the DNA fingerprinting, Dendrogram was constructed and genetic similarity matrix were estimated which revealed variations between selected species of Jasmine. A total of 15 random primers were used for conducting the RAPD analysis. The primers were OPZ5, OPZ6, OPZ7, OPZ8, OPZ9, OPZ10, OPZ11, OPZ12, OPZ13, OPZ14, OPZ15, OPZ18, OPZ19, OPZ20, OPZ21 and OPZ22. Of t hese selected random primers OPZ8, OPZ9, OPZ10 produced clear banding patterns which were used for constructing Dendrogram. OPZ8 produced a total 58 bands ranging from 7-11, OPZ9 produced a total 91 bands ranging from 10-13 and OPZ 10 produced a total 49 bands ranging from 5-8 for eight jasmine samples. Most of the bands were monomorphic with some polymorphic bands which can be used for marker development for these jasmine species. The described approach holds great promise for genetic diversity polymorphism, cultivar characterization and genetic population conservation of Jasmine species.

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