Growth Characteristics of Seven Hydrocarbon-Degrading Active Bacteria Isolated from Oil Contaminated Soil
Jinlan Xu, Yitao Zhang, Tinglin Hung and Hai xin Deng
Journal Title:Nature Environment and Pollution Technology
The growth characteristics of seven strains isolated from oil contaminated soil, as well as their respective degradation efficiency for various hydrocarbons were investigated. Factors that can impact biological oil degradation efficiency were revealed in a series of experiments. The results indicate that isolated strains could rapidly degrade crude oil, showing high activity in the first 13 h of bioremediation. These strains could grow in paraffin wax, which indicates that these strains could degrade long chain hydrocarbons. Some of them (SY22, SY23, SY24, SY42, SY43) were able to use short chain hydrocarbons and aromatic hydrocarbons as substrate, so these five strains are the preferred ones for the bioremediation of oil contaminated soil. Suitable pH for the growth of these five strains was in the range from 7 to 9. NH4NO3 and oil concentrations should range from 1000 mg/L to 1500 mg/L in order to achieve optimum conditions for petroleum hydrocarbon degradation. Adding organic matter such as starch and glucose accelerated oil and PAH degradation capability of the SY22, SY42 and SY23 strains. The presence of metal ions, such as Ni2+ and Co2+ in soil decreased the crude oil degradation efficiency of these strains, while metal ions, such as Fe2+ and Mn2+ did not affect the oil degradation activities.