Analysis of Microbial Community in the Anaerobic Phosphorus Sludge Using Molecular Techniques
Guanhua Gao, Hongwei Rong, Chaosheng Zhang, Kefang Zhang and Peilan Zhang
Journal Title:Nature Environment and Pollution Technology
Through anaerobic culture test, from the six different anaerobic phosphorus removal sludge, it was found that Anaerobic Sequencing Batch Reactor (ASBR) sludge is the most appropriate sludge source to remove phosphorus in the liquid medium, followed by Expanded Granular Sludge Bed (EGSB) sludge and chicken manure. Microbial community structures in the six different sludge sources were investigated by 16S Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) method when the anaerobic systems operated steadily. The DGGE fingerprints were analysed by the software Quantity One to obtain the information of the microbial species in the six different sludge sources, which showed that there was a high diversity in the bacterial communities, and the richness value of ASBR sludge was 0.59 whose number occupied more than half of the total bands, while The richness value reached highest (0.61) when it was chicken manure. The community similarity between ASBR sludge and EGSB sludge is the highest 71.7. In the bands strength schematic diagram, the No. 1, No. 8 and No. 18 band that existed in ASBR sludge, EGSB sludge and chicken manure might represent the colony related to the anaerobic phosphorus microbe.