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Studies on methionine secreting micro-organisms from sago industrial wastes and standardization of growth parameters for maximum methionine secretion

N.ARUNKUMAR*, S.P. SUNDARAM, R.ANANDHAM, N.O. GOPAL AND S. BALAKRISHNAN1

Journal Title:International Journal of Agricultural Sciences
Abstract


Methionine is a dietary essential amino acid secreted by various microorganisms and can be supplemented in food and feed for enhancing the growth and body functions of the mammals. From a total of141 microorganisms isolated from various sources such as curd, yogurt, cheese, and sago industrial waste 2 yeast culture and 2 bactreial cultures were confirmed to be elite methionine secreting organisms. The microorganisms through molecular characterization were identified as Candida tropicalis ITEM10456, Kluyveromyces marxianus CHY1612, Acetobacter tropicalis NRIC 0312, and Lactobacillus paracasei subsp. tolerans JCM 1171. Various parameters such as carbon and nitrogen requirement, optimum pH and temperature for maximum methionine secretion were also investigated. The organisms were found to secrete maximum methionine, Candida tropicalis ITEM10456 (1134 ?g/ml),Kluyveromyces marxianus CHY1612 (1320 ?g/ml), Acetobacter tropicalis NRIC 0312 (1412 ?g/ml), Lactobacillus paracasei subsp. Tolerans JCM 1171 (1078 ?g/ml) after standardization of the growth parameters

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