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In vitro regeneration in snake gourd (Tricosanthes anguina) from shoot apical meristem and young leaves

In vitro regeneration in snake gourd (Tricosanthes anguina) from shoot apical meristem and young leaves

M. E. HAQUE, D. REZWANA, M. I. ISLAM, AND B. SIKDAR DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology University of Rajshahi, Rajshahi-6205, Bangladesh

Journal Title:Journal of Crop and Weed
Abstract


Snake (Tricosanthes anguina) gourd is a popular vegetable and medicinal plant in Bangladesh. The present investigation was under taken for in vitro regeneration of snake gourd from shoot apical meristem and juvenile leaves in Murashige and Skoog (1962) medium supplemented with various concentration and combination of BAP, 2,4-D, NAA, IBA and GA3. Among different concentrations, 1 mg l-1BAP was found for effective callus induction from shoot apical meristem and 2.5 mg l-1 2, 4- D + 0.5 mg l-1BAP from leaves. The highest result for shoot regeneration was observed from meristem derived calli using 1.5 mg l-1BAP + 0.2 mg l-1GA3 and 2.5 BAP + 0.2 mg l-1GA3 from leaves derived calli. In vitro regenerated shoots were rooted well in ½ strength MS with 0.5 mg l-1NAA and well rooted plantlets were successfully established in pot soil with 80% survival rate.

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