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Paper Details

THE RATE OF BIOSYNTHESIS OF ISOLATED ACTIVE PRINCIPLE 4-[(5-CARBOXY-2-HYDROXY)-BENZYL]-1, 10-DIHYDROXY-3, 4, 7, 11, 11-PENTAMETHYLOCTAHYDROCYCLOPENTANAPHTHALENE FROM AN ANTARCTIC CYANOBACTERIUM NOSTOC CCC 537 UNDER DIFFERENT GROWTH REGIMES

Deepali*, Sreshwar P Singh, RK Asthana

Journal Title:World Journal of Pharmaceutical Research
Abstract


The known active principle (AP) 4-[(5 -carboxy -2-hydroxy)-benzyl]-1,10-dihydroxy -3,4,7,11,11-pentamethyloctahydrocyclopentanaphthalene has been isolated from an Antarctic cyanobacterium Nostoc CCC537, grows well at 20 oC and showed antibacterial properties against both Gram (+) and (-) bacteria as well as Mycobacterium tuberculosis. The Killing efficiency of AP is high when methanol used as a solvent as compared to water and DMSO. Even the biomass age of 60 day, the target cyanobacterium possess maximum antibacterial potential. To enhance rate of biosynthesis of AP, resuming the nutrient components of growth medium such as nitrate and phosphate. Under 29 M of phosphate as compared to 116 M, normal (58 M), the biomass production were reduced while rate of AP biosynthesis raised. Similarly for nitrate, biomass had increased maximum for 10mM while AP maximum in 5mM concentration. The production rate of AP were analyzed qualitatively by size of inhibition zones (Antibacterial bioassay) and quantitatively by HPLC.

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