IN VITRO SCREEENING OF PHYTOCHEMICALS AND ANTICANCER ACTIVITY OF ARGEMONE MEXICANA LEAF EXTRACT
Selvaraj Varun, Sudha Sellappa*
Journal Title:World Journal of Pharmaceutical Research
The present study investigates the qualitative and quantitative analysis
of the major bioactive constituents of Argemone mexicana in aqueous,
ethanol and methanol extract of root, stem and leaf. Phytochemical
analysis of the root, leaves and stem extracts showed the presence of alkaloids, phenol, sugar, terpenoids, glycosides, flavonoids and
tannins. The flavonoids and tannin contents were present in maximum level in the aqueous extract of A. mexicana leaf followed by stem and root extracts. MCF- 7 Breast cancer cells were treated with different
concentrations of the aqueous extract of leaf at two different time
intervals. Cell viability and apoptosis induction, were investigated.
A.mexicana extract treated MCF-7 cells exhibited a marked increase in apoptosis as compared to untreated group and the data showed that the
activity of caspase-3, -8 and -9 were significantly enhanced by A. mexicana treatment. Aqueous extract of the leaf showed dose-dependent cytotoxic and apoptotic effect in MCF-7 breast cancer cells. The phytochemical analysis of A.mexicana fetches pharmaceutical
companies in the production of the new drugs against cancer.