SEPARATION, PARTIAL PURIFICATION AND MITOGENICITY OF ENTERIC VIBRIO FLUVIALIS ANDAEROMONAS HYDROPHILA LIPOPOLYSACCHRIDES IN CHICKEN AND RAT
Dr.Ibrahim M S Shnawa*, Khalied Y Alzamily and Dr.Rabab Omran
Journal Title:World Journal of Pharmaceutical Research
Crude smooth LPS preparations were made from Vibrio fluvialis and
Aeromonas hydrophila. Then these preparations were processed in gel filteration using sepharose 50 resine which give two fractions from each of the test isolates that reach the limits of partial purity. Test for
an in vivo mitogenicity were carried out using chicken and rat models. The assessments were performed through skin induration and bone marrow lymphocyte blastogenicity. Fraction 1 and fraction 2 from each of the LPS preparations were proved to be mitogenic, Though F2
of the two LPS preparations were of higher mitogenicity than fraction 1.Such mitogenic potentials were stable and crossing the species barriers.