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Paper Details

ESTIMATION OF BETULINIC ACID IN EXTRACTS AND FORMULATIONS CONTAINING ALBIZIA LEBBECK BY HPLC

Pratima Tatke*, Sonal Desai, S. Y. Gabhe

Journal Title:World Journal of Pharmaceutical Research
Abstract


A triterpene acid, betulinic acid has been studied extensively because of its wide range of biological properties specially its anti -tumor activity. The present method discusses a new, simple and sensitive reversed phase HPLC-DAD method for quantitative estimation of betulinic acid in stem bark extract and formulations of Albizia lebbeck. Betulinic acid was analysed using MZ-Analytical C18 column (250 mm X 4.6 mm, 5 m) as stationary phase. Elution was carried out using acetonitrile: water (92:08, v/v) at flow rate of 1.0 ml/min. Detection was carried at 205 nm. Column oven temperature and injection volume were kept at 25 0 C and 20 l, respectively. Linearity of the proposed method was found to be over the range 0.3-60 g/ml. LOD and LOQ were found to be 0.1 g/ml and 0.3 g/ml, respectively. The method was found to be accurate with % recovery of 92.40 - 96.54 %.

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