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Paper Details

PCR BASED DETECTION AND ANTIBIOTIC RESISTANCE OF PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL AND WATER SAMPLES

*Wathiq Abbas Aldraghi

Journal Title:World Journal of Pharmaceutical Research
Abstract


700samples consist of 350 vaginal swaps from pregnant women in Kamal al-Samarrai hospital, Baghdad, Iraq and 350 of pool water samples . This study was carried out during January 2013 to January 2014 conducted by biochemical tests to diagnose the bacteria Pseudomonas aeruginosa and compare these results with the diagnosis by PCR technique based on gene gyr B by the special primer . The result was of the samples contain Pseudomonas aeruginosa , it was found that 50 positive sample (7.1%) with Pseudomonas aeruginosa , 10 isolates from clinical samples and 40 isolates from water samples. Distribution of age groups in relation to the clinical samples was as follows: The highest frequency of infection (62%) was observed at 3140 years followed by 4150 years (23%). The highest percentage rates of resistance were found for cefotaxim (100% of all isolates), nalidixic acid (75%), gentamicin (58.5%), and trimethoprim/ sulfamethoxazole (49%). Resistance rates were 24% or lower for piperacillin, kanamicin, cefaclore, ciprofloxacin and norfloxacin, and amikacin. A highly specific assay was developed by targeting gyrB gene for the detection of P. aeruginosa in inflammations, combined with optimized sample collection methods and sample processing, so the direct DNA extraction from vaginal swaps was possible while achieving the desired sensitivity of the method.

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