EVALUATION OF ENZYMATIC AND NON-ENZYMATIC ANTIOXIDANT ACTIVITIES OF IN VIVO AND IN VITRO REGENERATED ALOE VERA L.
Neelofar Khanam* and G.K. Sharma
Journal Title:World Journal of Pharmaceutical Research
Since ancient time a great number of aromatic, medicinal, spice and
other plants contain chemical compounds exhibiting antioxidant
properties, such as their secondary metabolites, which play an
important role in protecting the cell or tissues from oxidative damage.
In the present investigation in vitro enzymatic and non-enzymatic
antioxidant activities in the leaves of in vivo and in vitro regenerated
Aloe vera L. were tested. The plant leaves were analysed for the
selected enzymatic antioxidants such as superoxide dismutase (SOD),
catalase (CAT), peroxidase (POD), glutathione S-transferase (GST),
polyphenol oxidase (PPO), and for the non-enzymatic antioxidants
such as ascorbic acid, tocopherol, total carotenoids, total phenols, reduced glutathione,
flavonoids were examined. Results were showing that the leaves from in vitro propagated
Aloe vera L. were having higher level of antioxidants when compared to antioxidant levels in
the leaves of in vivo grown Aloe vera L.