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Paper Details

ASSESSMENT OF GENETIC DIVERSITY IN WATERMELON (CITRULLUSLANATUS (THUNB.) BY USING SDS-PAGETHROUGH GENETIC ANALYSIS SOFTWARE

Rajesh Goud Gajula*, Dr. Shailima RD Vardhini, Dr. Sujatha Edupuganti, G.Raghavendar

Journal Title:World Journal of Pharmaceutical Research
Abstract


Seed storage Protein profiles of five varieties of CitrullusLanatus were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total seed storage protein of Citrullus Lanatus were resolved on 10% SDS Polyarylamide gels showed variations in their banding pattern. The dendrogram constructed by NTSys PC software. The SAHN clustering based on UPGMA algorithms has put the all accession into different cluster groups. The Jaccords coefficient also calculated. Maharaja Variety and Super dragon verity has close genetic relation. Shahanshah and Maharaja have highest variation among five cultivars.Jaccards similarity shows that Maharaja and Shahanshah are 55% similar which is less similarity in all cultivars, Maharaja and Super Dragon are 73% similar, Maharaja and Durgesh has 73% similarity, Super Dragon and Durgesh are 71% similar. More work is needed to identify the specific genes in the cultivars, quantitative trait loci (QTL) works are carrying out to identify and correlate the genes for different traits using RAPD Technique.

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