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Paper Details

IDENTIFICATION OF CYMBOPOGON SPECIES BY USING RAPD TECHNIQUE

Parul Tripathi*, Dr. Aditi Singh

Journal Title:World Journal of Pharmaceutical Research
Abstract


Cymbopogon species shows a wide variation in morphological attributes & oil composition at inter & intraspecific levels. Knowledge of germplasm diversity is very important for plant conservation & improvement, therefore there is an interest in determining the genetic diversity in Cymbopogon germplasm. Although, morphological traits may be used to access genetic diversity as they are strongly influenced by environmental conditions & show little variation at the intraspecific level. Therefore, molecular markers are used to obtain much reliable estimates of genetic diversity. Amongst them, Random Amplified Polymorphic DNAs have been widely employed for the identification of ancestors, cultivars & discerning the genomic diversity in Cymbopogons. However, RAPDs have some limitations as molecular markers, the most important is being poor reproducibility of the profiles across the laboratories. The objective of the present study is to analyse genetic relationship as well as diversity amongst the three species of cymbopogon (winterianus, citrates, martinii). The three specieses are collected from approximately same geographical region of India. DNA after isolation amplified with specific forward & reverse primers & RAPD was performed. The study showed minor level of genetic diversity, which may be because they were from approximately same geographical region.

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