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Paper Details

EVALUATION OF ANTIDIABETIC AND ANTIOXIDATIVE EFFECT OF SEED OF EUJENIA JAMBOLANA IN STREPTOZOTOCIN INDUCED DIABETIC MALE RAT

Kishalay Jana, Abhinandan Ghosh, Tushar Kanti Bera, Debidas Ghosh*

Journal Title:World Journal of Pharmaceutical Research
Abstract


Present study focus on the effect of ethyl acetate fraction of seed of Eujenia jambolana (E. jambolana) in streptozotocin (STZ) induced diabetic male rats. Rats were divided into four groups (n=6), control, diabetic, ethyl acetate fraction treated (200 mg/kg body weight/day for 35 days) and glibenclamide treated (20 mg/kg body weight/day for 35 days) group. Fasting blood glucose levels (FBG) were measured of all animals. Important carbohydrate metabolic enzymes i.e. hexokinase, glucose-6-phosphatase and glucose-6-phosphate dehydrogenase were measured in hepatic tissue. Serum insulin, glycated hemoglobin (HbA 1 C) and glycogen content also measure in hepatic and skeletal muscle tissue. For antioxidant activity we measured catalase (CAT), peroxidase (Px) superoxide dismutase (SOD) along with the quantification of thiobarbituric acid reactive substances (TBARS) and conjugated diene (CD) in hepatic and renal tissue. Histological study of pancreatic tissue also evaluated. Serum glutamate oxaloacetate transaminase (GOT) and serum glutamate pyruvate transaminase (GPT) levels were measured for toxicity assessment. The FBG levels, serum insulin, HbA 1C, three carbohydrate metabolic enzymes activity and antioxidant enzymes along with lipid peroxidation end product were recovered significantly after fraction treatment in compare to untreated diabetic group. Degeneration of pancreatic islets cell were noted in untreated diabetic group, but after fraction treatment it was recovered towards the control animals. All results were compared with glibenclamide treated group, a standard antidiabetic drug. This study indicates that the ethyl acetate fraction of seed of E. jambolana is effective for the treatment of diabetes mellitus without any toxicity.

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