Isolation, identification, partial purification, optimization and characterization of alkaline protease fromNeisseria flavescence
SHRUTI SHUKLA AND O.P. VERMA
Journal Title:Asian Journal of Bio Science
Microbial alkaline proteases are among the important hydrolytic enzyme and have been used extensively since the advent of
enzymology. Bacterial extracellular alkaline proteases are of great importance due to its wide spectrum applications in detergent
industries, bioremediation, food industries, and leather processing and bio-film degradation. From the various niches eighteen
isolates were screened for alkaline protease production, out of which four isolates showed efficient alkaline protease production.
Out of four bacterial species one of the isolate i.e. N. flavescence showed significant enzyme activity. Optimization of the pH and
temperature conditions for enzyme production was determined and was found to be 7.0 and 600C, respectively. Optimization of
carbon, nitrogen sources and metal ions for enzyme production were determined and was found to be 4.64U/ml for sucrose, 0.91U/
ml for gelatin and 0.21U/ml for zinc chloride for the isolates. The yield of alkaline protease was inhibited by copper sulphate.
Enzyme activity was assayed using tyrosine-casein method. The purified enzyme preparations of having enzyme activity 0.26U/
ml was also excellent in destaining of ink colour. The molecular weight of different bands of alkaline protease fromN. flavescence
ranged from 25kD to 83kD.