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Paper Details

A SIMPLE AND RAPID METHOD FOR THE QUANTIFICATION OF IMATINIB MESYLATE AND DESMETHYL IMATINIB IN HUMAN PLASMA USINGLC-MS/MS AND ITS APPLICATION TO ROUTINE THERAPEUTIC DRUG MONITORING

Jose Francis* Biswajit Dubashi Rajan Sundaram Suresh Chandra Pradhan Adithan Chandrasekaran

Journal Title:World Journal of Pharmaceutical Research
Abstract


Introduction: Imatinib mesylate is the standard of care in the treatment of chronic myeloid leukemia. Therapeutic drug monitoring (TDM) can be used as a tool to cross-check treatment adherence or toxic manifestations to imatinib mesylate in chronic myeloid leukemia patients. The study aimed to develop a simple, cost effective and rapid method for quantification of imatinib mesylate and desmethyl imatinib in human plasma using LC-MS/MS. Methods: The biological samples were prepared by a simple protein precipitation method and separated using an XTerra MS C18 column. Imatinib and its metabolite desmethyl imatinib was quantified using multiple reaction monitoring (MRM) with a triple quadrupole tandem mass spectrometer working in the positive electrospray ionization (ESI+) mode using verapamil as internal standard. RESULTS: The method was validated and found to be precise and accurate within the dynamic range of 5ng-8g/mLfor the drug and 5ng-1g/mL for the metabolite. The method was then applied to routine therapeutic drug monitoring of CML patients and the meanSD trough level concentration was found to be 1437459 ng/mL. CONCLUSION: This simple and rapid LC-MS/MS method was validated to be sensitive, specific, precise and accurate for thequantification of imatinib and its metabolite in human plasma. The method was found to be cost effective for implementing in routine TDM process.

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