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Paper Details

ANTIOXIDANT ACTIVITY OF PAPAIN HYDROLYSATES OF OYSTER MUSHROOM (PLEUROTUS OSTREATUS) PROTEIN

Dr.Sundaram Meignanalakshmi*, Huldah,J., Palanisammi. A and Dhinakar Raj,G

Journal Title:World Journal of Pharmaceutical Research
Abstract


In the present study Oyster mushroom (Pleurotus ostreatus) protein has been isolated and concentration of protein was found to be 12.4mg/ml by biuret method. The total protein was hydrolysed by papain. 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and superoxide free radical scavenging activity of total protein and papain hydrolysate were analysed. Papain hydrolysate was found to be having highest 2,2-diphenyl-1-picrylhydrazyl and superoxide free radical scavenging activity and was purified by using HPLC. Out of four fractions obtained, fraction 2 was found to be having highest 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity of 93.69% and 93.44% of superoxide free radical scavenging activity at 1mg/ml concentration.

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