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Paper Details

REGULATION OF GLUCOSE TRANSPORTERS IN CELL MEMBRANE DURING DIABETES: IMPACT OF FLAXSEED OIL SUPPLEMENTATION

Jihan Hussein*, Zakaria El-Khayat, Olfat Shaker, Dina Abo El-Matty, Wafaa Rasheed, and Jakleen Raafat

Journal Title:World Journal of Pharmaceutical Research
Abstract


Insulin resistance is associated with cell membrane properties . Some studies have demonstrated relationships between fatty acids composition of cell membrane phospholipids and insulin action. This study aimed to improve cell membrane structure ( increasing the omega-3 fatty acids ) in order to decrease insulin resistance through regulation of glucose transporters. Forty male albino rats were used in this study and divided into four groups .Group I (control group): healthy rats received a vehicle. Group II (flaxseed oil group): healthy rats received 1.2 ml flaxseed oil / kg b.w. / day orally. Group III (diabetic group): diabetic rats received a vehicle. Group IV (treated group): diabetic rats received 1.2 ml flaxseed oil / kg b.w. / day orally . Fasting blood sugar and plasma insulin were determined .Erythrocyte membrane fatty acids were estimated by HPLC column C 18 ( 260 X 4.6 , particle size 5 ?l ) , mobile phase was acetonitrile / water mixture (70/30) v/v by isocratic elution with flow rate 1 ml / min and 214 nm wave length. GLUT 1 and GLUT 4 were estimated by PCR . The current results indicated that, the percent of changes of GLUT1 and GLUT4 in treated group from diabetic were 18.42 and 44.64 % respectively; also a negative correlation was observed between insulin resistance and glucose transporters. In conclusion, flaxseed oil supplementation has an important role in improving the cell membrane structure and elevation of glucose transporters resulting in a reduction of insulin resistance during diabetes.

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