BIOACTIVITY AND COMPOSITION OF THE FLOWERS OF SPATHODEA CAMPANULATA P. BEAUV
Naglaa G. Shehab*, Hanaa H. Eid and Soheir M. El Zalabani
Journal Title:World Journal of Pharmaceutical Research
Among plant metabolites, phenolics were found to possess a wide
spectrum of biological activities. The purpose of this study was to
investigate the ethanol extract of the flowers of Spathodea
campanulata P. Beauv. (EEFSC) in terms of bioactivity and
composition. Special emphasis was made on its phenolic components.
of EEFSC (up to 4 g/kg) indicated its safety. The growth
inhibitory activity of EEFSC, and its hexane, chloroform, ethyl acetate
and n-butanol fractions was evaluated in-vitro against a set of microorganisms and 2 human tumor cell lines (breast and colon). The
chloroform fraction exhibited the highest antibacterial effect and the
against both cell lines. The anthelmintic and acute anti-hyperglycemic
potentialities of EEFSC were also explored and found noticeably significant. RP-HPLC with
UV detection was employed for identification and quantification of the floral phenolics. Six
compounds were isolated from the active chloroform and ethyl acetate/n-butanol fractions.
The purified isolates included three phenolics, and were identified as phytol, ?-methylcinnamaldehyde, ?-sitosterol -3-acetate, naringenin, catechin-3-O-?-rhamnopyranoside
and 5, 6, 4' trihydroxy flavonol-7-O-?-rhamnopyranoside, all being for the first time reported
from the flowers. The observed bioactivities were correlated to the isolated constituents.