ISOLATION, SCREENING AND OPTIMIZATION OF NOVEL LACCASE PRODUCTION FROM PENICILLIUM CHRYSOGENUM
Ganiya,R and Anuradha,R*.
Journal Title:World Journal of Pharmaceutical Research
The present work focuses on isolating, screening and optimizing the
process parameters to achieve the maximum production of
extracellular laccases by penicillium chrysogenum obtained from
marine soil. Guaiacol was used as substrates for screening the laccase
activity. Culture condition like pH, temperature and nutritional
parameter like carbon source were optimized. The optimization studies
revealed that the laccase yield was highest when operated at pH 6.0
tempherature 60?C and carbon source-maltose in the production
medium. The present study showed that relatively simple plate test
screening method can be used for discovery of novel laccases.