ROLE OF GRAPE SEED AND SKIN EXTRACT IN PREVENTION OF DOXORUBICIN INDUCED CARDIOTOXICITY IN RAT MODEL
Dr. Subinay Datta* and Dr. Mrinal Pal
Journal Title:World Journal of Pharmaceutical Research
Background: Doxorubicin is a potent chemotherapeutic drug The
clinical usefulness of doxorubicin has been limited largely by the risk
of cardiomyopathy and life-threatening heart failure. Cellular changes
leading to this toxicity are suggested to be mediated through a druginduced increase oxidative stress. Grapeseed Proanthocyanidin [GSP]
seeds possessing a potent antioxidant properties Aim: So, the present
study was conducted to investigate possible protective effects of Grape
seed and skin extract in doxorubicin-induced cardiotoxicity in rat
model. Method: The male Wister rats (n = 34) were randomly selected
and divided into three groups. The control group (n = 12) received
distilled water [1ml/kg body weight] orally for 15 days, second group (n = 10) rats were
injected intra peritoneal with a single dose of Doxorubicin (10mg/kg) in normal saline and in
third group Doxorubicin (10mg/kg) was administered intraperitoneally and pretreatment with
Grape seed and skin extract extract (200mg/kg body weight) before one hour DOX treatment
by oral gavage for 15 consecutive days. Result: It was revealed by elevated serum cardiac
biomarkers in comparison of control and associated with increasing levels of myocardial
malondialdehyde [MDA] with simultaneously increase in the level of Superoxide dismutase.
On daily oral administration of aqueous suspension of GSP seeds extract in the dose of (200
mg\kg ) for 15 days produced normalization in the serum levels of heart marker enzymes.
Conclusion: The present study has demonstrated that Grape seed and its skin has got definite
potency to ameliorate oxidative stress and Doxorubicin induced cardiotoxicity.