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Paper Details


Gurjeet Singh*, Raksha, R.K. Mukhia, A.D. Urhekar

Journal Title:World Journal of Pharmaceutical Research

Background: Laboratory surfaces harbor many microbial pathogens as the patient samples are kept on laboratory surfaces during processing. The aim of the study was to find out bacterial and fungal pathogens on laboratory surfaces of various sections of Microbiology laboratory Bacteriology, Mycology, Serology, media preparation and discard section. This study will help design methods to help in prevention of laboratory acquired infection among laboratory workers and others who transport the samples from patients to laboratory and also collect the reports. Material and Methods: This prospective study was conducted in a microbiology laboratory of Mahatma Gandhi Hospital, Navi Mumbai, India. The period of study was 6 months from November 2013 to April 2014. Samples were collected from - various surfaces of microbiology laboratory with moistened (pre-moistened with sterile peptone water) two cotton swabs. One swab was inoculated onto MacConkey agar, Blood agar, and Chocolate agar media and incubated at 37C for 24 to 48 hours and other inoculated on Sabourauds dextrose agar media and incubated for 1 to 7 days at 25-28C. Results: We isolated and identified various bacteria and fungi from these surfaces. In our study , the distribution of microorganisms on laboratory surfaces were Bacillus species 36.36% followed by Coagulase negative Staphylococcus 14.29% Staphylococcus aureus 12.99%, Diptheroids 10.39%, Micrococcus 9.09%, Pseudomonas aeruginosa and Klebsiella species 6.49% each, Aspergillus species 2.60% and Candida species 1 1.30% were isolated. Conclusions: Our study showed that all areas of the laboratory are contaminated with pathogenic / non pathogenic bacteria and fungi. It indicates that these are a potential source of transmission of infection from the hands of laboratory workers to themselves / others if proper precaution is not taken.