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Paper Details

Isolation of root nodule bacteria from Trigonella foenum graceium

POOJA AGRAWAL*, VIDESH PATERIYA1, CHHAYA NAGAR2 AND ALOK GARG3

Journal Title:International Journal of Agricultural Sciences
Abstract


Wild legumes were collected from three different places of Patkoi village, district Sagar. A total of 27 bacteria were isolated from 3 different samples of wild leguminous plant Trigonella foenum graceium. The nodules of wild methi (Trigonella) yielded bacteria other then Rhizobium with different cultural characteristics. Only eight isolates were circular with exopolysaccharide secretion rest were showing different colony characteristics. The colonies were white in colour and the elevation were found to be convex also called as pelvinate colony. In citrate utilization test all isolates found to be negative except the isolates 13B and W07. Where as all isolates were able to grow at alkaline pH 11 but showed poor growth at acidic pH 4. W07 isolate were given positive result for indole production while remaining 7 isolates were negative. Isolates 13B and W07 shown positive result while tested for 3-ketolactose production

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