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Paper Details

Evaluating the Efficacy of Olive, Apple and Grape Seed Extracts in Reducing Escherichia coli O157:H7 Contamination on Organic Leafy Greens during the Wash Process

Buddhini P. K1, Jones J2, Ravishankar S3, Jaroni D1*

Journal Title:International Journal of Food Science, Nutrition and Dietetics (IJFS)

Powdered extracts of apple, olive, and grape seed were evaluated for antimicrobial activity against Escherichia coli O157:H7 during processing and subsequent short-term storage of organic leafy greens. The leafy greens tested were, baby spinach, mature bunched spinach, romaine lettuce, and iceberg lettuce. The organic leafy greens were thoroughly washed and inoculated with a three-strain cocktail of E. coli O157:H7 (6 log CFU ml-1). Antimicrobial treatments of the extracts, prepared in phosphate buffered saline, at 1, 3, and 5% concentrations were used to wash contaminated leafy greens which were then stored for 3 days at 4C. Surviving E. coli O157:H7 populations were enumerated on days 0, 1, and 3 of storage. Significant reductions (P