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Paper Details

Preparation and Characteristic of Dextran-BSA Antibody and Establishment of its Elisa Immunoassay

Zhen-ming X1*, Li-sha F1, Da-feng L1,2, Lin Y1

Journal Title:International Journal of Food Science, Nutrition and Dietetics (IJFS)
Abstract


The enzyme linked immunosorbent assay (ELISA) is a potential tool for the determination of dextran. In this study, dextranBSA antigens were prepared by Reductive amination method, and were confirmed by SDS-PAGE and free amino detection. The effects of coupled reaction conditions such as different oxidation degree of dextran, the reaction time were investigated and the immunity of the resulting dextran-BSA neoglycoprotein antigens were evaluated through the interaction with standard dextran antibody. The immunogen was immunized with white rabbits to obtained polyclonal antibody respectively. A general and broad class-specific Elisa detection method was developed according to Elisa theory. The method was put to use for quantitative analysis of dextran in practical saccharose samples.

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